Regulating and changeable performance of CDX2, CTNNBIP1, and FAT4 genes in colorectal cancer

Abstract

Introduction: Colorectal cancer (CRC) is the third most frequent type of cancer in the world. In this explanation, genetic variation is associated in all cancers, particularly CRC, and modifications of numerous genes, such as CDX2, CTNNBIP1, and FAT4, are linked to tumorgenesis in CRC. As a result, this research was conducted in order to determine changes in the expression of these genes.

Materials and Methods: After obtaining patient consent and pathology department approval, from72 individuals with confirmation of pathology report,were provided and bought from the Bio banks. Real-time PCR was used to examine the expression of CDX2, CTNNBIP1, and FAT4 genes in tumoral and non-tumoral tissues. These genes' histological associations with grading and staging for upregulation and downregulation were examined.

Result: CDX2 (P = 0.01) and CTNNBIP1 (P = 0.03) expression were highly increased, whereas FAT4 (P= 0.05) expression was downregulated. Similarly, there was no evidence of a link between CDX2 and CTNNBIP1 overexpression and grade, stage, lymphnode metastasis, or distant metastasis. Furthermore, FAT4 expression was linked to  highe stage, high grade, distant metastasis and lymphnode metastasis (P 0.05).

Conclusion: CTNNBIP1 and CDX2 genes were upregulated in tumoral tissues, while FAT4 genes were downregulated. Finally, changes in the expression of these genes can be used as a CRC biomarker.