Importance of QF-PCR method in aborted embryos in comparison with other common relative determination aneuploidies methods

Authors

  • Kosar Babaei Non-Communicable Disease Research Center, Neyshabur University of Medical Sciences, Neyshabur, Iran
  • Mohsen Azimi-Nezhad Non-Communicable Disease Research Center, Neyshabur University of Medical Sciences, Neyshabur, Iran 2 / UMR INSERM U 1122, IGE-PCV, Interactions Gène-Environment En Physiopathologie Cardiovascular Université De Lorraine, Nancy, France
  • Ali Akbar Samadani Clinical Research Development Unit of Poursina Hospital, Guilan University of Medical Sciences, Rasht, Iran / Healthy Ageing Research Center, Neyshabur University of Medical Sciences, Neyshabur, Iran

Keywords:

QF-PCR, Aneuploidies, Abortion, Chromosomal abnormalities

Abstract

One of the most important method in cytogenetic in order to diagnose the chromosomal abnormalities, is QF-PCR (Quantitative fluorescent polymerase chain reaction).In this way, QF-PCR can be employed  in diagnosing the chromosome duplication number by amplification of repeat sequences at polymorphic loci. These repeat sequences are amplified by PCR, and the labelled yields are classified by gel electrophoresis method. Importantly, QF-PCR reaction has been in diagnostic application in many countries and has confirmed to be a robust, cost-effective, and precise rapid prenatal test for many types of common aneuploidies. Special benefits comprise detection of mosaicism, triploidy and maternal cell contamination. So, we try to declare the importance of this technique in comparison with other ones in this review article.

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Published

2021-09-19

How to Cite

Babaei, K., Azimi-Nezhad, M. ., & Samadani, A. A. . (2021). Importance of QF-PCR method in aborted embryos in comparison with other common relative determination aneuploidies methods. Journal of Current Oncology and Medical Sciences, 1(1), 65–79. Retrieved from http://submission.journalofcoms.com/index.php/JCOMS/article/view/12

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